Candidate molecules affecting the initiation of tooth germ
Head: RNDr.
Mária Hovořáková, PhD.
Principal investigator of the project: Mgr. Linda
Dalecká
Co-investigator of the project:
Mgr.
Klára Steklíková
Mgr.
Zuzana Pavlíková
Anotation:
Tooth development, similarly to other ectodermal derivates, is a multifactorial process based on interactions between epithelium and mesenchyme. To study this process in vertebrates a mouse dentition model is commonly used, because of the similarity to the human dentition during its embryonic development. Dental placodes are presented in upper and lower jaws in the mouse embryo in the places for prospective incisors and molars, and the tooth budding is initiated here by the expression of specific genes. It is not completely clear, how these places are defined. With the aim of searching for the candidate molecules, which may influence the dental initiation, we plan to use RNA sequencing from the area in the cheek region of the lower jaw, where the budding will start in the future. We will focus on the most relevant molecular changes, which can occur in these areas in very early stages of embryonic development. Candidate molecules will be then used to influence the areas of the embryonic diastema using in vitro cultivation and we will evaluate their effect in the tissue during the development. Except of the contribution to the knowledge in the field of odontogenesis a deeper understanding of the principal of tooth initiation is important for understanding general organogenesis and also to explain some of the dental pathologies.