Laboratory of Experimental Embryology focuses on the general early embryonic development and on molecular signallings during prenatal development of teeth and other cranial structures, as well as of limbs and heart with special focus on Sonic hedgehog pathway. In frame of our research topics, we take advantage from routine usage of transgenic mouse models, cell lineage tracing experiments, RNA sequencing and candidate gene analysis, in situ hybridization and immunostaining We combine advanced in-vitro culturing on the mouse and reptilian tissue and in-ovo cultures on chick with modern imaging technologies. Our developmental biology topics overlap regenerative medicine knowledge and offer gaining of new insights into developmental processes essential for discovering healing technologies and treatment methods.
Head:
RNDr. Mária Hovořáková, PhD.
Contact:
Albertov 4, Praha 2, 128 00
E-mail: maria.hovorakova@lf1.cuni.cz
Phone: 224968128
Research scientists:
Prof. Abigail S. Tucker
RNDr. Mária Hovořáková, PhD.
Ph.D. Students:
Linda Šulc Dalecká, MSc.
Zuzana Pavlíková, MSc.
Klára Steklíková, MSc.
Lucie Horáková, MSc.
Pregraduate students:
Veronika Sejrková
Tereza Smlsalová
Tereza Svobodová
Technician:
Ivana Koppová
Recent grant support:
GA ČR, 23-06660S Physiological dynamics of odontogenesis in context of surrounding structures (2023-2025)
GA ČR, 21-04178S - Integrating Sprouty function with FGF and primary cilia during development. (2021 - 2023)
GA UK, 1312820 - Candidate molecules affecting the initiation of tooth germ. (2020 - 2022)
Cooperation:
King's College, London, UK
Laboratoire de Biologie et Modelisation de la Cellule, Lyon, France
Medical University of Vienna, Austria
Faculty of Natural Sciences of Comenius University in Bratislava, Slovak Republic.
Third Faculty of Medicine, Prague, Czech Republic.
Institute of Animal Physiology and Genetics, The Czech Academy of Sciences, Brno, Czech Republic.
Karolinska Institute in Stockholm, Sweden.
Max-Planck-Institut für Evolutionsbiologie, Plon, Germany
Papers:
2023:
Qiu T, Hutečková B, Seppala M, Cobourne MT, Chen Z, Hovořáková M, Buchtová M, Tucker AS. Molecular profiling of the vestibular lamina highlights a key role for Hedgehog signalling. Development. 2023 Apr 1;150(7):dev201464. doi: 10.1242/dev.201464. Epub 2023 Apr 6. PMID: 36971701.
Hedvičáková V, Žižková R, Buzgo M, Vištejnová L, Klein P, Hovořáková M, Bartoš M, Steklíková K, Luňáčková J, Šebová E, Paurová I, Rysová M, Filová E, Rampichová M. The Gradual Release of Alendronate for the Treatment of Critical Bone Defects in Osteoporotic and Control Rats. Int J Nanomedicine. 2023 Feb 1;18:541-560. doi: 10.2147/IJN.S386784. PMID: 36756052; PMCID: PMC9901358.
Horakova L, Dalecka L, Zahradnicek O, Lochovska K, Lesot H, Peterkova R, Tucker AS, Hovorakova M. Eda controls the size of the enamel knot during incisor development. Front Physiol. 2023 Jan 9;13:1033130. doi: 10.3389/fphys.2022.1033130. PMID: 36699680; PMCID: PMC9868551.
2022:
Groborz O, Kolouchová K, Pankrác J, Keša P, Kadlec J, Krunclová T, Pierzynová A, Šrámek J, Hovořáková M, Dalecká L, Pavlíková Z, Matouš P, Páral P, Loukotová L, Švec P, Beneš H, Štěpánek L, Dunlop D, Melo CV, Šefc L, Slanina T, Beneš J, Van Vlierberghe S, Hoogenboom R, Hrubý M. Pharmacokinetics of Intramuscularly Administered Thermoresponsive Polymers. Adv Healthc Mater. 2022 Sep 25:e2201344. doi: 10.1002/adhm.202201344. Epub ahead of print. PMID: 36153823.
2021:
Lavicky J, Kolouskova M, Prochazka D, Rakultsev V, Gonzalez-Lopez M, Steklikova K, Bartos M, Vijaykumar A, Kaiser J, Porizka P, Hovorakova M, Mina M, Krivanek J. The development of dentin microstructure is controlled by the type of adjacent epithelium. J Bone Miner Res. 2021 Nov 16. Epub ahead of print. PMID: 34783080. doi: 10.1002/jbmr.4471.
Hruba E, Kavkova M, Dalecka L, Macholan M, Zikmund T, Varecha M, Bosakova M, Kaiser J, Krejci P, Hovorakova M, Buchtova M. Loss of Sprouty produces a ciliopathic skeletal phenotype in mice through upregulation of Hedgehog signaling. J Bone Miner Res. 2021 Aug 23. doi: 10.1002/jbmr.4427. Epub ahead of print. PMID: 34423857.
2020:
Hayden L, Lochovska K, Sémon M, Renaud S, Delignette-Muller ML, Vilcot M, Peterkova R, Hovorakova M, Pantalacci S. Developmental variability channels mouse molar evolution. Elife. 2020 Feb 12;9:e50103. doi: 10.7554/eLife.50103.
Hovorakova M, Zahradnicek O, Bartos M, Hurnik P, Stransky J, Stembirek J, Tucker AS. Reawakening of Ancestral Dental Potential as a Mechanism to Explain Dental Pathologies. Integr Comp Biol. 2020 Sep 1;60(3):619-629. doi: 10.1093/icb/icaa053.
Qiu T, Teshima THN, Hovorakova M, Tucker AS. Development of the Vestibular Lamina in Human Embryos: Morphogenesis and Vestibule Formation. Front Physiol. 2020 Jul 16;11:753. doi: 10.3389/fphys.2020.00753. eCollection 2020.
2019:
Sadier A, Twarogowska M, Steklikova K, Hayden L, Lambert A, Schneider P, Laudet V, Hovorakova M, Calvez V, Pantalacci S. Modeling Edar expression reveals the hidden dynamics of tooth signaling center patterning. PLoS Biol. 2019 Feb 7;17(2):e3000064. doi: 10.1371/journal.pbio.3000064. eCollection 2019 Feb.
Newton PT, Li L, Zhou B, Schweingruber C, Hovorakova M, Xie M, Sun X, Sandhow L, Artemov AV, Ivashkin E, Suter S, Dyachuk V, El Shahawy M, Gritli-Linde A, Bouderlique T, Petersen J, Mollbrink A, Lundeberg J, Enikolopov G, Qian H, Fried K, Kasper M, Hedlund E, Adameyko I, Sävendahl L, Chagin AS. A radical switch in clonality reveals a stem cell niche in the epiphyseal growth plate. Nature. 2019 Mar;567(7747):234-238. doi: 10.1038/s41586-019-0989-6. Epub 2019 Feb 27.
Vesela B, Svandova E, Hovorakova M, Peterkova R, Kratochvilova A, Pasovska M, Ramesova A, Lesot H, Matalova E. Specification of Sprouty2 functions in osteogenesis in in vivo context. Organogenesis. 2019;15(4):111-119. doi: 10.1080/15476278.2019.1656995. Epub 2019 Sep 4.
2018
Hovorakova M, Lesot H, Peterka M, Peterkova R. Early development of the human dentition revisited. J Anat. 2018 Aug;233(2):135-145. doi: 10.1111/joa.12825. Epub 2018 May 10.
Kaucka M, Petersen J, Tesarova M, Szarowska B, Kastriti ME, Xie M, Kicheva A, Annusver K, Kasper M, Symmons O, Pan L, Spitz F, Kaiser J, Hovorakova M, Zikmund T, Sunadome K, Matise MP, Wang H, Marklund U, Abdo H, Ernfors P, Maire P, Wurmser M, Chagin AS, Fried K, Adameyko I. Signals from the brain and olfactory epithelium control shaping of the mammalian nasal capsule cartilage. Elife. 2018 Jun 13;7:e34465. doi: 10.7554/eLife.34465.